The phic31 integrase system for gene therapy
Webb2 okt. 2024 · This was mainly achieved by mutating HIV-1 integrase at functional hotspots of the ... Taken together, these results show that the … WebbThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA …
The phic31 integrase system for gene therapy
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Webb28 feb. 2014 · The therapeutic potential of PhiC31 integrase as a gene therapy system. Expert Opin. Biol. Ther. 11, 1287–1296, 10.1517/14712598.2011.601293 (2011). Article … Webb28 feb. 2012 · In order to select relevant systems to evaluate new therapies, ... Gene therapy for cystic fibrosis: an example for lung gene therapy. Gene Ther. 2004; 11 suppl. 1:S43–S50. [PMC free article ... Rudolph C. Phage phiC31 integrase-mediated genomic integration and long-term gene expression in the lung after nonviral gene delivery. J ...
Webb• Knock in & out using TALEN system and phiC31 Integrase system. • Lead the project in supporting development based on cell engineering. Scientist WebbThe potential use of the φC31 integrase system in gene therapy opens up the possibilities of new treatments for old diseases. φC31 integrase mediates the integration of plasmid …
Webb4 mars 2016 · The first studies utilizing phiC31 integrase for gene therapy took advantage of a relatively simple and effective in vivo delivery method in mice, hydrodynamic … WebbThe phiC31 integrase may represent one of the best hopes for nonviral gene therapies for RA. This article provides evidence that phiC31 integrase generates stable transgene expression in cultured synoviocytes and rabbit joints, illustrating the potential of this nonviral vector to be used for RA gene therapy. 9.
Webb1 juni 2011 · It is demonstrated that in the presence of the phiC31 integrase, precise unidirectional integration occurs with an efficiency of 100% in Escherichia coli and >50% …
WebbDNA has been a main focus of biomedical research ever since its discovery as the hereditary molecule. This discovery laid the foundation for scientist… greater st. bethel church middlesex ncWebbThe φC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The φC31 integrase can integrate introduced plasmid DNA into … flintstones daughter\u0027s nameWebb30 aug. 2011 · The therapeutic potential of phiC31 integrase as a gene therapy system. Introduction: The φC31 integrase system is a phage-derived system that offers the … flintstones daughterWebbThe PhiC31 Integrase System is an non-viral, integrase-based gene expression system for low- or single-copy integrations up to large insert sizes. The PhiC31 Systems comes in a … greater statesville chamber of commerceWebbTransgene delivery systems, particularly those involving retroviruses, often result in the integration of multiple copies of the transgene throughout the host genome. Since site-specific silencing of trangenes can occur, it becomes important to greater staten island veterinary servicesWebb2 apr. 2009 · PhiC31 integrase facilitates efficient integration of transgenes into human and mouse genomes and is considered for clinical gene therapy. However recent … greater steal pathfinderWebb20 mars 2024 · The phiC31 integrase is a member of serine-catalyzed enzymes encoded by this phage and mediates the aforementioned phage genome insertion(Smith and … flintstones decals