WebJun 20, 2005 · When Mg·ATP and herring sperm DNA (Promega) were included as iron-binding molecules, saturating amounts were used (1 mM and 12.5 μg/ml, respectively, determined by saturation of their effects on the reaction rate). At each time point, aliquots were added to 0.01% catalase and 400 μM ferrozine in a 50 mM sodium phosphate buffer … WebFeb 15, 2008 · DNA extraction from all sample matrices was performed using the Wizard Plus Miniprep DNA purification system (Promega, Madison, USA). Usually 200 mg of ground sample material was extracted and DNA was eluted in 50 μl elution buffer according to the supplier’s manual. ... Herring sperm DNA-solution (20 ng/μl) was used as diluent. These ...
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WebNov 14, 2024 · A sperm DNA methylation biomarker assay has been developed and validated, which uses a microarray approach to assess CpG islands within the genome 15. … WebDec 1, 2001 · Decorator probes were hybridized as described, except that 0.1 mg/ml herring sperm DNA was included in the hybridization reaction and the incubation at 37°C was for 2 h. The slides were then washed with 2× SSC, 0.05% Tween-20 twice for 2 min, rinsed in 2× SSC for 2 min and dried at 55°C before scanning. pap gennevilliers mail
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WebSpecifications For Use With (Application) Chromatin Biology, Nucleic Acid Gel Electrophoresis, Blotting Key Functions Block Non-Specific Hybridization Product Line UltraPure™ Product Type Herring Sperm DNA Solution … WebCostar (Cambridge, MA). Purified genomic herring sperm DNA (catalog number D-1816) was purchased from Promega (Madison, WI) and Tris and EDTA were obtained from Sigma-Aldrich (St Louis, MO). Several series of dilutions of purified herring sperm DNA were made using TE buffer (10 mM Tris and 1 mM EDTA, pH 8.0) as the diluent. WebForensic DNA Analysis (MPS) Equipamentos Bioquímicos CE Instruments Fluorimetros + Luminómetros Automação Diagnóstico Molecular Diagnóstico Molecular Microsatellite Instability Testing Análise de Proteínas Immunoassays Espectrometria de Massas Quantificação de Proteínas + Detecção Expressão de Proteínas Interação de Proteínas paph airport