Lamina joint inclination assays

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WebApr 3, 2024 · BL has been isolated from various dicot plants, and although bioassays such as lamina joint inclination assays show that BL has strong BR activity in rice, ... Muto T and Todoroki Y Brassinolide-2,3-acetonide: A brassinolide-induced rice lamina joint inclination antagonist. Bioorg Med Chem. 2013;21: 4413–4419. pmid:23673217 . View … WebFeb 5, 2024 · For lamina joint inclination assay, sterilized rice seeds were grown in the dark at 30 °C for 9 days. We obtained equally sized samples by excising approximately 2 …

Rice Lamina Joint Inclination Assay - PubMed

WebJul 20, 2024 · To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we … WebLamina joint inclination assay in wild type and OsBLR1-transgenic lines. a, c and e The lamina joint inclination assay using different concentration of eBL in blr1-D, BLR1-OE … domestic appliance repairs barnsley

The basic helix-loop-helix transcription factor OsBLR1 ... - Springer

WebA rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in the concentration range of 5 x 10- 5 Jlg/ml to 5 x 10- 3 Jlg/ml, when uniform seedlings of the rice WebDec 5, 1998 · It has been found that pretreatment with 0.1 and 1 μg of IAA before application of brassinolide increased activity by 10- and 100-fold, respectively, in the rice lamina … WebJan 1, 2024 · Leaf angle is determined by lamina joint inclination and is an important agronomic trait that determines plant architecture, photosynthetic efficiency, and crop yield. Cytokinins (CKs) are phytohormones involved in shaping rice (Oryza sativa L.) architecture, but their role in leaf angle remains unknown. domestic appliance repairs bracknell

RIP2 interacts with REL1 to control leaf architecture by ... - Springer

The basic helix-loop-helix transcription factor OsBLR1 regulates …

WebBrassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR … WebApr 14, 2024 · Furthermore, a BR-induced lamina joint inclination assay (Wada et al., 1984) confirmed that the sensitivity of OsMYB7 overexpressors and the osmyb7-1 mutant are not altered by BR treatments, compared to the WT (Supplementary Figure S6). Taken together, these findings suggest that OsMYB7 is involved in the regulation of leaf angles … domestic appliance repairs barrow in furnessWebMar 8, 2024 · The leaf bending caused by extracts from Distylium racemosum in the rice lamina inclination assay is one of the earliest reports of brassinolide (1) activity in plants . In this assay, 1 caused dose-dependent swelling of the adaxial cells in the joint between the leaf blade and sheath of etiolated rice seedlings. domestic appliance recycling

"WebJul 3, 2014 · Leaf angle (lamina inclination or lamina joint bending) is the degree of bending between the leaf blade and leaf sheath or the inclination between leaf blade and culm. ... The assays were conducted as described previously (Zhang et al. 2012). Agrobacterium-mediated transfection of N. benthamiana leaves and polyethylene glycol … " - Lamina joint inclination assays

Lamina joint inclination assays

Abscisic Acid Represses Rice Lamina Joint Inclination by

WebFigure 3. BR-induced lamina joint inclination in WT (Hwayoung cultivar) and OsBUL1 knock-out mutant (osbul1) rice. The photo was taken after BL (10-6 M) treatment for 2 … WebMar 18, 2024 · Scanning electron microscopy revealed that the expansion of cell size in the adaxial side of the lamina joint was responsible for the increase in leaf angle in osbhlh079-D. The expression of...

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WebDec 1, 2015 · The rice lamina joint inclination assay is an extremely sensitive assay for detecting BR activity, based on measuring the angles between the lamina and the … WebJul 20, 2024 · To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we …

WebFigure 5. Measurement of lamina angles using the ImageJ program. The three points are marked by green arrows using the angle tool. The measured angle is marked by the box … WebNov 29, 2024 · We functionally characterize a novel rice SPOC domain-containing protein leaf inclination 3 (LC3), whose deficiency (lc3 mutant) presents enhanced leaf angle due to the excessive cell elongation at adaxial side of lamina joint, and demonstrate that LC3 controls leaf inclination by regulating auxin signaling through interacting with LIP1, a HIT ...

WebJan 27, 2024 · Lamina joint inclination test To test the sensitivity of ZH11, rel1, rip2, RIP2 -OE, and rel1rip2 to 24-epibrassinolide (24-epiBL) in a lamina joint inclination experiment, ethanol containing 0, 10, 100, or 1000 ng of 24-epiBL was applied to the lamina joint of the first leaf of rice seedlings. WebLamina joint inclination, coleoptile elongation, and root elongation assay results indicated that these overexpression lines were more sensitive to BR, while the knockout mutants were less sensitive. There was no significant difference in the endogenous BR contents of blr1-1/2 and wild-type plants.

WebMar 18, 2024 · The lamina joint, which connects the leaf blade and leaf sheath, is central in controlling leaf angle [ 4 ], as the degree of leaf inclination largely depends on cell proliferation or cell expansion as well as the cell wall composition at the lamina joint.

WebMay 12, 2024 · (G) Compared to ZH11, elt1-D or elt1/ELT1-RNAi seedlings display increased or reduced lamina joint inclination of etiolated leaves under 24-eBL treatment. Eight-day-old seedlings were analyzed and ... fake toy gun sniperWebAug 1, 2009 · The lamina joint bending assay is established as an indicator to evaluate the effects of plant hormones, herbi- cides and light on rice leaf bending, which is thought to affect crop yields (... domestic appliance repairs bodminWebMar 9, 2024 · Lamina joint inclination assays showed that the lamina joint bending angles of the T 1 progenies from the uORF OsBRI1 (−99, 28 aa)#14 were smaller than … domestic appliance repairs bury st edmundsWebApr 14, 2024 · Furthermore, a BR-induced lamina joint inclination assay (Wada et al., 1984) confirmed that the sensitivity of OsMYB7 overexpressors and the osmyb7-1 mutant … domestic appliance repairs derby fake town nuke testsWebJan 29, 2016 · For the lamina inclination assays, 1 µl of ethanol containing a series of concentrations of BRs, IAA or Brz was spotted onto the tips of the lamina of 5- or 6-day-old seedlings. C-22-hydroxylated and 6-deoxo brassinosteroids were identical to those described by Nakamura et al. (2009). fake toyota partsWebLamina joint inclination or leaf angle (the angle between the leaf blade and vertical culm) is a major trait of rice plant architecture. The plant hormone brassinosteroid (BR) is the … fake towns on maps